We are a young interdisciplinary group working at the interface of Chemistry and Biology. Our research activities are focused on mechanistic understanding of various metallo-enzymes and their applications. Employing various tools of chemistry, biochemistry and molecular biology we are exploring function, kinetics, mechanism and reaction intermediates of several poorly understood enzymes. We are also very keen to engineer and utilize these enzymes in green energy applications and therapeutics.

Representative Publications

• Membrane Association of Monotopic Phosphoglycosyl Transferase Underpins Function^†
  Ray, L. C.^‡; Das, D.^‡; Entova, S.; Lukose, V.; Lynch, A. J.; Imperiali, B.^*; Allen, K. N.^*
  Nature Chem. Biol. 2018, 14, 538-541.
   
  DOI: 10.1038/s41589-018-0054-z
  
  ^†This article has been highlighted by C&EN magazine, and recommended by F1000Prime as being of special significance. ^‡ Co-first authors
• Analysis of a Dual Domain Phosphoglycosyl Transferase Reveals a Ping-Pong Mechanism with a Covalent Enzyme Intermediate^†
  Das, D.; Kuzmic, P; and Imperiali, B.^*
  Proc. Natl. Acad. Sci. U.S.A., 2017, 114, 7019-7024.
   
  DOI: 10.1073/pnas.1703397114
  
  ^†This article has been recommended by F1000Prime as being of special significance
• A Rapid and Efficient Luminescence-based Method for Assaying Phosphoglycosyltransferase Enzymes^†
  Das, D.; Walvoort, M. T. C.; Lukose, V.; and Imperiali, B.^*
  Sci. Rep., 2016, 6, 33412.
   
  DOI: 10.1038/srep33412
  
  ^†This article has been highlighted by Promega
• Mechanistic Insights from Reaction of α-Oxiranyl Aldehydes with Cyanobacterial Aldehyde Deformylating Oxygenase
  Das, D.; Ellington, B.; Paul, B.; and Marsh, E. N. G.^*
  ACS ChemBio., 2014, 9, 570-577.
   
  DOI: 10.1021/cb400772q
• Probing the Mechanism of Cyanobacterial Aldehyde Decarbonylase Using a Cyclopropyl Aldehyde
  Paul, B.^‡; Das, D.^‡; Ellington, B.^‡; and Marsh, E. N. G.^*
  J. Am. Chem. Soc., 2013, 135, 5234-5237
  DOI: 10.1021/ja3115949
  
  ^‡ Co-first authors
• Oxygen-independent Decarbonylation of Aldehydes by Cyanobacterial Aldehyde Decarbonylase: a New Reaction of Di-iron Enzymes.
  Das, D.^‡; Eser, B. E.^‡; Han, J.; Sciore, A.; and Marsh, E. N. G.^*
  Angew. Chem. Int. Ed., 2011, 50, 7148-7152
  DOI: 10.1002/anie.201101552
  
  ^‡ Co-first authors
• Anti-thyroid Drug Carbimazole and its Analogues: Synthesis and Inhibition of Peroxidase-Catalyzed Iodination of L-Tyrosine
  Das, D.; Roy, G.; Mugesh, G.^*
  J. Med. Chem., 2008, 51, 7313-7317
   
  DOI: 10.1021/jm800894m